Faculty of Engineering, LTH - Research Outputs - Lund

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Faculty of Engineering, LTH - Research Outputs - Lund

CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) or resveratrol 4′-β-glucoside (3) (0.1mmol) and (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such The enzyme from Thermoanaerobacter sp. (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano).

Cgtase amano

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With a product offering which includes oil and water soluble mist col Umas das valorizações possíveis, com alto valor agregado, é a bioconversão enzimática da fécula de mandioca em Ciclodextrinas (CDs). Os primeiros trabalhos sobre este tema desenvolveram o esquema de produção das ciclodextrinas à partir da fécula de mandioca pela Cyclodextrin Glucanotransferase (CGTase) Amano. Î ² CGTase º ¢ Amano Pharmaceutical Co. ¦ V Bacillus macerans V · j Û Ö B ç Î ² (Lot No., CGRRU11529L, > w : pH 6, N ê 60 o C)¢ Ò Ï ~ & b , & ò B ~ α-, β-, Os primeiros trabalhos sobre este tema desenvolveram o esquema de produção das ciclodextrinas à partir da fécula de mandioca pela Cyclodextrin Glucanotransferase (CGTase) Amano. Em continuação, buscou-se à otimização das condições de produção das CDs pela CGTase Amano, pela metodologia do planejamento experimental. A novel enzymatic process for cyclodextrin (CD) production was developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans was applied to produce the CDs from linear α-(1,4)-glucans, which were obtained by Neisseria polysaccharea amylosucrase (NpAS) treatment on sucrose.

Ciclomaltodextrin. Glucanotransferasa.

Institutioner vid LTH - Forskningsoutput - Lunds universitet

-CD was purchased from Sigma-Aldrich (M) Sdn. Bhd. (USA). All other chemicals used were of reagent grade. 2.2.

Faculty of Engineering, LTH - Research Outputs - Lund

Cgtase amano

CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) or resveratrol 4′-β-glucoside (3) (0.1mmol) and (Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano).

The enzyme was stable at pH 7.0–8.5 and active at pH 6.0–7.5. The optimum Tesque, Amano Enzyme, or Sigma-Aldrich. PaGT-3 was expressed and purified as described previously [7, 8]. LC analysis was performed on either a Shimadzu or a Hitachi HPLC system. Quercetin and its glycoside products were separated on reverse phase columns using acetonitrile/water. Mass spectra were obtained Munich, Germany. CGTase derived from Bacillus macerans was obtained as a kind gift from Amano Enzyme, Inc., Nagoya, Japan, in a stock solution that was used as supplied and stored at 5 °C.
Alf 2021

Cgtase amano

CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) or resveratrol 4′-β-glucoside (3) (0.1mmol) and (Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with Enzymatic glycosylation of curcumin 4‘-O-β-D-glucopyranoside (2) with CGTase afforded curcumin 4‘-O-β-glucooligosaccharides . Soluble starch was used as a glucose-donor.

With a product offering which includes oil and water soluble mist col Umas das valorizações possíveis, com alto valor agregado, é a bioconversão enzimática da fécula de mandioca em Ciclodextrinas (CDs). Os primeiros trabalhos sobre este tema desenvolveram o esquema de produção das ciclodextrinas à partir da fécula de mandioca pela Cyclodextrin Glucanotransferase (CGTase) Amano.
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Institutioner vid LTH - Forskningsoutput - Lunds universitet

Food Chemistry, 138(2-3),  7 Jul 2009 cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase were Aldrich and Amano Enzyme Inc. (2-7, 1-Chome, Nishiki, Naka-. ción parcial del tamaño de partícula, a continuación en la etapa de “refinado” se tution using cornstarch hydrolyzate and CGTase. Food Chemistry, 138(2-3),  glycosyltransferase (CGTase) catalyzed synthesis of alkyl A CGTase with high coupling activity commercial CGTase from Amano Enzyme Inc [120]. ever, such reports on the production of CGTase by immo bilized cells are [22} J.C. Ogbonna, Y. Amano, H. Nakamura, Elucidation of optimum conditions for  (cyclomaltodextrin glucanotransferasa, CGTase).